Link to the complete list of our publications.


eLife2014
Jonkers I*, Kwak H*, Lis JT. (2014) Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons. eLife. 3:e02407. (* equal contribution)

We analyzed how fast the RNA polymerase moves along the gene to synthesize RNA. This speed can be a critical checkpoint in controlling the amount of transcription and co-transcriptional RNA processing, but has only been measured in a small number of genes. Here, we used a chemical inhibitor of P-TEFb kinase that prevents RNA polymerase  escape from the promoter to visualize the clearance of RNA polymerase along the gene. By analyzing the the time-course of this clearance, we measured the elongation rates of up to a thousand genes at once.

 

 


Buckley MS*, Kwak H*, Zipfel WR, Lis JT. (2014) Kinetics of promoter Pol II on Hsp70 reveal stable pausing and key insights to its regulation. Genes Dev. 28(1):14-19. (* equal contribution)GenesDev2014

We showed that the RNA polymerase at the promoter are stably paused, although they are not infinitely stable and can be terminated. This termination was a consequence of increased turnover of RNA polymerase at the promoter rather than a control mechanism during heat-shock response in a major Drosophila heat-shock gene Hsp70.

 


AnnRevGenet2013Kwak H, Lis JT. (2013) Control of transcriptional elongation. Ann Rev Genet. 47:483-508.

This is a broad-spectrum review paper on the mechanisms of RNA polymerase elongation, and the factors affecting this process of productive RNA synthesis.

 


Science2013Kwak H, Fuda NJ, Core LJ, Lis JT. (2013) Precise maps of RNA polymerase reveal how promoters direct initiation and pausing. Science. 339(6122):950-3.

This paper demonstrates PRO-seq and PRO-cap methods to map genome-wide RNA polymerases and their start sites in base-pair resolution. We identified different modes of RNA polymerase pausing, and showed how promoter DNA sequences direct early transcription in Drosophila cells.