Single cell and spatial genomics

Our lab was one of the early adopters of single cell transcriptomics technologies in Cornell and provided expertise to our collaborators (see Johnson et al, Nature 2018; Part el al, Cell Rep. 2020). We are using this expertise to further develop novel genomic methods that can resolve the spatial contexts of cell-to-cell interactions in higher dimensions. This ultimate fusion of next-gen sequencing with 3D microscopy will be used to answer the following. 

Major questions

• How does cell-to-cell and cell-to-matrix interactions direct global changes in transcriptional landscape?
• Can oncogenic transcriptional reprogramming happen as a consequence of immune-cancer interaction?
• Elucidate the spatial context of epigenetic reprogramming in dental stem cell differentiation.